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      <image:title>Blog - SDS-PAGE gel with two resolving % - Make it stand out</image:title>
      <image:caption>Figure 1. Left: Coomassie staining of the 15:8% gel after transfer shows the gel interface near the middle of the gel and a rip where I pulled on the gel to try to separate the two gels but only ripped the 8% gel instead. Right: coomassie blue staining of the membrane after blotting.</image:caption>
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      <image:title>Blog - SDS-PAGE gel with two resolving % - Make it stand out</image:title>
      <image:caption>Figure 2. Left: image of the 14:8.5% 2:1 gel still on top of the membrane right after wet transfer showing the interface above the 100 kDa marker. Right: anti-mouse secondary HRP exposure showing Pgk1 and residual ubiquitin primaries superimposed on the marker layer (separate channel) by GeneSys software. The marker is the same as in Figure 1.</image:caption>
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